Molecular Sciences Bldg
21, James Cook University,
Telephone: 61-7-4781 5567
Fax: 61-7-4781 6078
| The SSBG
interests are broadly based on the study at the molecular level of
protein-ligand interactions and in particular protein-DNA interactions.
The study and discovery of these interactions is primordial to the
understanding of the complex spatio-temporal molecular communication
patterns occurring within the cell. The discovery and study of novel
interactions can provide new molecular tools with applications in the
field of bio- and nanotechnology (e.g. molecular diagnostics, drug
discovery, functional genomics and proteomics).
The SSBG is currently developing innovative methods for the discovery,
characterization and quantification of such interactions. The SSBG has
current collaborations with the research groups of Prof. Dixon (U of
Wollongong), Prof. Otting (ANU Canberra), Dr Oakley (CSIRO Mebourne),
Dr Sevick (ANU, Canberra) Dr Van Oijen (Harvard Medical School,
Boston), Prof. Strazewski (Université Claude-Bernard, Lyons,
and Prof. Keene (James Cook University). The research undertaken in the
SSBG is multidisciplinary and spans across the disciplines of Molecular
Biology, Biochemistry and Chemistry.
The SSBG is grateful for the generous support of the National Health
and Medical Research Council of Australia.
- Patrick M.
Schaeffer, DEUG, Licence, Maitrise, DEA, PhD
- Isabelle Morin - Postdoctoral Fellow
- Sam Askin - Research Associate
- Morgane Moreau - PhD Student 2009 - Current
- Nikki Freedman - PGDipResMeth - 2010
- Dahdah Dahdah - PGDipResMeth
- Zeinab Wehbi - Current
- Sarah Gierz - Summer Scholar 2009
IN THE SUPRAMOLECULAR BIOLOGY GROUP:
Students interested in undertaking a PhD or Masters project in the
Supramolecular Biology Group should contact Patrick Schaeffer to
of mutual interest. Most higher degrees students would be expected to
be eligible for a scholarship through the Australian
Postgraduate Award (APA) scheme, the James
Cook University Postgraduate Research Scholarship or an equivalent
national or international
The Australian Postgraduate Award (APA) and James Cook University
Postgraduate Research Scholarship (JCUPRS) are open to applicants with,
or who expect to hold, a first class honours degree or equivalent by
the end of the year and who wish to undertake full-time research
Masters or PhD program. In 2010 the stipends for an APA were $22,500 pa
(full time) or $12,176 (part-time). APA applicants must be Australian
citizens or have been granted permanent resident status and lived in
Australia continuously for 12 months prior to receiving the award;
Information and application forms can be obtained from the Graduate
Research School. Closing Date: 31 October of each year.
Students who have completed their undergraduate training in a BSc,
BBiomedSc, BMedlabSc or equivalent program and are interested in
the Biochemistry and Molecular Biology Honours Program are encouraged
Schaeffer for a
description of currently available projects. Honours studies require a
full-time commitment for one year (two semesters) and can start in
either February or August.
There are two main opportunities for undergraduates to participate in
the ongoing research of the Supramolecular Biology Group.
Students enrolled in BC3203 (Special Topics in Biochemistry and
Molecular Biology - second semester) could ask that they undertake
their research project in the Supramolecular Biology Laboratory.
Students can apply for a Comparative Genomics Centre Vacation
The successful applicants receive instruction in the latest recombinant
and genetics techniques, receiving a stipend of $200 per week for a
full-time commitment of between 6 and 10 weeks over the summer break.
Applications for the CGC Vacation Studentships are
announced in September each year and close in late October.
Schaeffer for further
Moreau, Morgane J.J., Morin, Isabelle, and Schaeffer, Patrick M.
determination of protein stability and ligand binding using a green
fluorescent protein reporter system. Molecular BioSystems, 6 (7).
pp. 1285-1292. ISSN 1742-206X
Morin, Isabelle, Dixon, Nicholas E., and Schaeffer, Patrick M. (2010) Ultrasensitive detection of
antibodies using a new Tus-Ter-lock immunoPCR system. Molecular
BioSystems, 6 (7). pp. 1173-1175. ISSN 1742-2051
Dahdah, Dahdah B., Morin, Isabelle, Moreau, Morgane J.J., Dixon,
Nicholas E., and Schaeffer, Patrick M. (2009) Site-specific covalent
attachment of DNA to proteins using a photoactivatable Tus–Ter complex.
Chemical Communications, 21 . pp. 3050-3052. ISSN 1364-548X
Schaeffer, Patrick, and Dixon, Nicholas (2009) Synthesis and applications of
covalent protein-DNA conjugates. Australian Journal of Chemistry,
62 (10). pp. 1328-1332. ISSN 1445-0038
Tanner, Nathan A., Hamdan, Samir M., Jergic, Slobodan, Loscha, Karin
V., Schaeffer, Patrick M., Dixon, Nicholas E., and van Oijen, Antoine
M. (2008) Single-molecule
studies of fork dynamics in Escherichia coli DNA replication.
Nature Structural and Molecular Biology, 15 (2). pp. 170-176. ISSN
Watt, Stephen J., Urathamakul, Thitima, Schaeffer, Patrick M.,
Williams, Neal K., Sheil, Margaret M., Dixon, Nicholas E., and Beck,
Jennifer L. (2007) Multiple
oligomeric forms of Escherichia coli DnaB helicase revealed by
electrospray ionisation mass spectrometry. Rapid Communications in
Mass Spectrometry, 21 . pp. 132-140. ISSN 1097-0231
Su, Xun-Cheng, Schaeffer, Patrick M., Loscha, Karin V., Gan, Pamela H.
P., Dixon, Nicholas E., and Otting, Gottfried (2006) Monomeric solution structure
of the helicase-binding domain of Escherichia coli DnaG primase.
FEBS journal, 273 (21). pp. 4997-5009. ISSN 1742-4658
Ioannou, Charikleia, Schaeffer, Patrick M., Dixon, Nicholas E., and
Soultanas, Panos (2006) Helicase
binding to DnaI exposes a cryptic DNA-binding site during helicase
loading in Bacillus subtilis. Nucleic Acids Research, 34 (18). pp.
5247-5258. ISSN 0305-1048
Mulcair, Mark D., Schaeffer, Patrick M., Oakley, Aaron J., Cross,
Hannah F., Neylon, Cameron, Hill, Thomas M., and Dixon, Nicholas E.
(2006) A molecular
mousetrap determines polarity of termination of DNA replication in E.
coli. Cell, 125 (7). pp. 1309-1319. ISSN 1097-4172
Beck, Jennifer L., Urathamakul, Thitima, Watt, Stephen J., Sheil,
Margaret M., Schaeffer, Patrick M., and Dixon, Nicholas E. (2006) Proteomic dissection of DNA
polymerization. Expert Review of Proteomics, 3 (2). pp. 197-211.
Graham, Stephen C., Lilley, Penelope E., Lee, Mihwa, Schaeffer, Patrick
M., Kralicek, Andrew V., Dixon, Nicholas E., and Guss, J. Mitchell
(2006) Kinetic and
crystallographic analysis of mutant Escherichia coli aminopeptidase P:
insights into substrate recognition and the mechanism of catalysis.
Biochemistry, 45 (3). pp. 964-975. ISSN 1520-4995
Robinson, Andrew, Wu, Peter S.-C., Harrop, Stephen J., Schaeffer,
Patrick M., Dosztanyi, Zsuzsanna, Gillings, Michael R., Holmes, Andrew
J., Nevalainen, K. M. Helena, Stokes, H. W., Otting, Gottfried, Dixon,
Nicholas E., Curmi, Paul M. G., and Mabbutt, Bridget C. (2005) Integron-associated mobile
gene cassettes code for folded proteins: the structure of Bal32a, a new
member of the adaptable α+β barrel family. Journal of Molecular
Biology, 346 (5). pp. 1229-1241. ISSN 0022-2836
Oakley, Aaron J., Loscha, Karin V., Schaeffer, Patrick M., Liepinsh,
Edvards, Pintacuda, Guido, Wilce, Matthew C.J., Otting, Gottfried, and
Dixon, Nicholas E. (2005) Crystal
and solution structures of the helicase-binding domain of Escherichia
coli primase. Journal of Biological Chemistry, 280 (12). pp.
11495-11504. ISSN 1083-351X
Schaeffer, Patrick M., Headlam, Madeleine J., and Dixon, Nicholas E.
(2005) Protein - protein
interactions in the eubacterial replisome. IUBMB Life, 57 (1). pp.
5-12. ISSN 1521-6551
Molecular biology, biochemistry,
biological chemistry, bio-inorganic chemistry, biotechnology,
diagnostics, ELISA, biacore, surface plasmon resonance, structure,
protein interactions, associations, dissociations, affinity, binding,
DNA replication, transcription factor, Tus, DnaB, primase, polymerase,
SSB, single molecule, protein stability, unfolding, aggregation, green
fluorescent protein, chromatin immuno-precipitation, ImmunoPCR,
real-time PCR, Escherichia coli, Bacillus, sortase, artificial
nuclease, in vivo localization, proteomics, functional genomics,
structural genomics, directed evolution, purification.lar science,